Contact an Account ManagerComparative determination of bacitracin by HPLC and microbiological methods in some pharmaceuticals and feed grade preparations
Summary
Type: HPLC
Application #: 103750
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: UV, 220 nm
Conditions: eluent: aqueous phase a) 0.01M KH2PO4, b) 0.05M KH2PO4, organic phase methanol - acetonitrile (1:1), isocratic elution with aqueous phase - organic phase (51:49, v/v)
flow rate 1.4ml/min
Authors: Pavli V
Source: J Liquid Chromatogr 13 (1990) 303 - 318
Details
Legend: Fig. 2. Influence of KH2PO4 concentration in the mobile phase on the separation
of Zn-bacitracin working standard.